A simple, rapid and precise reverse phase high performance liquid chromatography method was developed for the analysis of Almotriptan. Chromatographic separation of Almotriptan was performed by using a waters C18 column (250 x 4.6 mm, 5 µm) as stationary phase with a mobile phase comprising of Methanol, ACN, Acetic acid 75:20:5 (v/v/v) at a flow rate of 1.0 ml/min and UV detection at 231 nm. The linearity of Almotriptan is in the range of 20 ppm to 120 ppm. The limit of detection for Almotriptan was found to be 0.2 ppm. The proposed method was found to be accurate, precise and rapid for the analysis of Almotriptan. Degradation test was also performed.
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